navigation
Deutsch English Home Service Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie

   LabMed Letters
   Patient Info
   Quality Management
   Research
   Websites  

nav_footer
nav_dekoline
Home → Service → Research → Correlation of cell division and specific protein production during the course of lymphoid cell differentiation


Correlation of cell division and specific protein production during the course of lymphoid cell differentiation

Wolf D. Kuhlmann, M. Bouteille, S. Avrameas

Immunocytochemistry SFB 136 and Institut für Nuklearmedizin DKFZ Heidelberg, BRD;
Laboratoire de Pathologie Cellulaire, Centre des Cordeliers (Université de Paris VI),
75270 Paris Cx 06, France,
Unité d’Immunocytochimie, Département de Biologie Moléculaire,
Institut Pasteur, 75015 Paris, France



Mice were immunized with horseradish peroxidase and injected with [3H]thymidine. Popliteal lymph node cells were submitted to electron microscopic immunocytochemistry and high resolution autoadiography in order to correlate antibody production and ability to undergo cell division at various stages of lymphoid cell differentiation. Antibody synthesis started in blast cells and increased steadily until the mature plasma cell stage was reached. Thymidine incor-poration was highest in blastoid cells and decreased continuously afterwards. Chromatin dispersion was found to be paralleled by thymidine incorporation. Almost all mature plasma cells were devoid of thymidine incorporation. This confirms that they are end cells apparently unable to divide.

download