navigation
Deutsch English Home Service Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie

   LabMed Letters
   Patient Info
   Quality Management
   Research
   Websites  

nav_footer
nav_dekoline
Home → Service → Research → Glucose oxidase as label in histological immunoassays with enzyme-amplification in a two-step technique: coimmobilized horseradish peroxidase as secondary enzyme for chromogen oxidation


Glucose oxidase as label in histological immunoassays with enzyme-amplification in a two-step technique: coimmobilized horseradish peroxidase as secondary system enzyme for chromogen oxidation

Wolf D. Kuhlmann, P. Peschke

Ernst-Rodenwaldt-Institut, D-5400 Koblenz, Germany
Institut für Nuklearmedizin, DKFZ, D-6900 Heidelberg, Germany



A sensitive staining procedure for glucose oxidase (GOD) as marker in immunohistology is described. The cytochemical procedure involves a two-step enzyme method in which GOD and horseradish peroxidase (HRP) are coimmobilized onto the same cellular sites by immunological bridging or by the principle of avidin-biotin interaction. In this coupled enzyme technique, H2O2 generated during GOD reaction is the substrate for HRP and is utilized for the oxidation of chromogens such as 3,3-diaminobenzidine or 3-amino-9-ethylcarbazole. Indirect antibody labelled and antibody bridge techniques including the avidin (streptavidin)-biotin principle have proven the usefulness of this GOD labelling procedure for antigen localization in histological sections.

download