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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
Reagents, solutions
Laboratory methods
Acronyms
References
Applied cell labeling  

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HomeCell-MarkerLaboratory methods → HRP cytochemistry




HRP cytochemistry

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany



A range of substrates is available for the cytochemical staining of peroxidase activity. The most utilized substrates are diaminobenzidine for brownish color, aminoethylcarbazole for reddish reactions and chloronaphthol to give a blue-black product. Thus, in double or triple staining experiments, antigens can be subsequently stained in the same tissue section.

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