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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
Reagents, solutions
Laboratory methods
Acronyms
References
Applied cell labeling  

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HomeCell-MarkerReagents, solutions → Washing solutions




Washing solutions

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany



Between the various immunohistological incubation steps, cell preparations (sections, cytospins, cell cultures etc.) must be treated by washing solutions in order to take off unreacted substances which might interfere with reagents added in subsequent stages. The type of washing as well as the type of appropriate washing solution will depend on the material under study. We give here a selection of the most applied washing buffers for routine immunohistology.

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