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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
Reagents, solutions
Laboratory methods
Acronyms
References
Applied cell labeling  

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HomeCell-MarkerTissue preparation → Microtomy of tissue specimens, collection of sections




Microtomy of tissue specimens, collection of sections

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany



Histological sections are cut from embedded tissues and placed on glass slides. This is done with a microtome. Paraffin blocks are cut with steel blades. Plastic embedded tissue blocks are cut with glass or diamond knife. Frozen sections prepared with a cryo-microtome are sometimes necessary either for rapid histopathological diagnostics or as an alternative to paraffin histology when for a number reasons special methods cannot be performed with the latter. When tissue sections will not adhere sufficiently strong to glass slides, adhesives are recommended. The most popular way to keep sections on slides is to coat them with polylysine (a basic polymer) or with APES (3-aminopropyltriethoxysilane).

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