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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
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Applied cell labeling  

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HomeCell-MarkerApplied cell labeling → Diaminobenzidine cytochemistry in cryo-ultramicrotomy for the detection of peroxidases




Diaminobenzidine cytochemistry in cryo-ultramicrotomy for the detection of peroxidases

Wolf D. Kuhlmann, A. Viron

Immunocytochemistry SFB 136, Institut für Nuklearmedizin, Deutsches Krebsforschungszentrum
Im Neuenheimer Feld 280, D-6900 Heidelberg, Germany
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France



Rat erythrocytes and lymphoid cells were fixed by glutaraldehyde and encapsulated in bovine serum albumin plus rabbit IgG globulins for cryo-ultramicrotomy. A technical procedure is described by which endogenous peroxidases of erythrocytes in ultrathin frozen sections were detected by hydrogen peroxide and diaminobenzidine as hydrogen donor. Modifications of this classical cytochemical procedure proved also useful in cryo-ultramicrotomy for immunoperoxidase labeling of antigenic determinants in rabbit IgG globulins which have been cross-linked within the supporting matrix of cells.

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