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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
Reagents, solutions
Laboratory methods
Applied cell labeling  

HomeCell-MarkerMicroscopy and stains in histology → Immuno enzyme techniques in cytochemistry

Immuno enzyme techniques in cytochemistry

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany

Histological stains are commonly used to make cell structures more readily visualized than in unstained specimens. They serve to reveal structural compounds and differences during evolution or in pathological conditions. Classical histological dyes possess only restricted specificity depending on their selectivity to bind with cellular components. The demand for highly specific stainings of molecules at the cellular level has initiated the development of dedicated probe techniques. Such techniques are either of histochemical nature or they are based on specific ligand bindings e.g. antibodies, lectins, nucleotides. For the location of those bindings appropriate detection systems are used.