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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
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Laboratory methods
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Applied cell labeling  

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HomeCell-MarkerLaboratory methods → Azur-methylene blue staining of resins sections




Azur-methylene blue staining of resins sections

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany



Histological staining of semithin sections is readily achieved with Azure B and methylene stains. This procedure was originally introduced by KC RICHARDSON et al. (1960) and gives an excellent information of the tissue morphology. Azure B is formed by the oxidation of methylene blue and used in Azure-eosin stains of blood smears. Methylene blue is a commonly used dye in histology. Following ‘ripening’ which is a process of atmospheric or chemical oxidation, the three metachromatic dyes Azure A, B and C are formed (polychrome methylene blue).

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