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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
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Laboratory methods
Acronyms
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Applied cell labeling  

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HomeCell-MarkerLaboratory methods → Staining with Alcian blue at pH 2.5




Staining with Alcian blue at pH 2.5

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany



Two major types of mucins can be readily distinguished by histochemical methods, i.e. acid and neutral mucins (glycoconjugates). A significant advance in the histological detection of mucins was achieved by the introduction of stainings such as the periodic acid-Schiff (PAS) and Alcian blue at pH 2.5, Alcian blue at pH 1.0 and high iron-diamine/Alcian blue as staining agents. Alcian blue 8GX is primarily used for the detection of acid mucins.

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