navigation
Deutsch English Home Service Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie Bitte wählen Sie

Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
Reagents, solutions
Laboratory methods
Acronyms
References
Applied cell labeling  

nav_footer
nav_dekoline
HomeCell-MarkerLaboratory methods → Toluidine blue staining




Toluidine blue staining

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany



Subsequent to immunohistological reaction, counterstaining of tissue sections with the thiazin dye toluidine blue (Toluidine blue O) is an alternative to other dyes (e.g. haematoxylin, carmine, methyl green) for nuclear staining. Moreover, the staining procedure is rapid and easily performed, thus, toluidine blue is helpful and widely employed for any kind of overview stainings. There exist formulations for cryostat and paraffin sections as well as for sections cut from resin embedded tissue.

download